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| Status |
Public on Jul 18, 2023 |
| Title |
Optimized minimal genome-wide human sgRNA library |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
To minimize the human genome-wide CRISPR/Cas9 library size, we established H-mLib which recruited a novel sgRNA design method and applied with dual plasmid based strategy.
The performance of the H-mLib was benchmarked to other CRISPR libraries in a proliferation screening conducted in K562 cells.
We also identified human core essential genes and cell-type specific essentials genes in K562 and Jurkat cells.
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| Overall design |
CRISPR screen
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| Contributor(s) |
Zhou Y, Wang L, Lu Z, Yu Z, Ma L |
| Citation(s) |
37464007 |
| Submission date |
Jan 17, 2023 |
| Last update date |
Sep 12, 2023 |
| Contact name |
Lijia Ma |
| E-mail(s) |
malabdata@westlake.edu.cn
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| Organization name |
Westlake university
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| Department |
School of Life Sciences
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| Lab |
Lijia Ma
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| Street address |
Shilongshan Road No.18, Cloud Town, Xihu District
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310024 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (51)
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| GSM6939410 |
K562 cells, Brunelllo CRISPR/Cas9 library, day0, replicate 1 |
| GSM6939411 |
K562 cells, Brunelllo CRISPR/Cas9 library, day0, replicate 2 |
| GSM6939412 |
K562 cells, Brunelllo CRISPR/Cas9 library, day4, replicate 1 |
| GSM6939413 |
K562 cells, Brunelllo CRISPR/Cas9 library, day4, replicate 2 |
| GSM6939414 |
K562 cells, Brunelllo CRISPR/Cas9 library, day10, replicate 1 |
| GSM6939415 |
K562 cells, Brunelllo CRISPR/Cas9 library, day10, replicate 2 |
| GSM6939416 |
K562 cells, Brunelllo CRISPR/Cas9 library, day18, replicate 1 |
| GSM6939417 |
K562 cells, Brunelllo CRISPR/Cas9 library, day18, replicate 2 |
| GSM6939418 |
K562 cells, Brunelllo CRISPR/Cas9 library, day24, replicate 1 |
| GSM6939419 |
K562 cells, Brunelllo CRISPR/Cas9 library, day24, replicate 2 |
| GSM6939420 |
K562 cells, H-mLibA CRISPR/Cas9 library, day0 |
| GSM6939421 |
K562 cells, H-mLibA CRISPR/Cas9 library, day4, replicate 1 |
| GSM6939422 |
K562 cells, H-mLibA CRISPR/Cas9 library, day4, replicate 2 |
| GSM6939423 |
K562 cells, H-mLibA CRISPR/Cas9 library, day10, replicate 1 |
| GSM6939424 |
K562 cells, H-mLibA CRISPR/Cas9 library, day10, replicate 2 |
| GSM6939425 |
K562 cells, H-mLibA CRISPR/Cas9 library, day18, replicate 1 |
| GSM6939426 |
K562 cells, H-mLibA CRISPR/Cas9 library, day18, replicate 2 |
| GSM6939427 |
K562 cells, H-mLibA CRISPR/Cas9 library, day24, replicate 1 |
| GSM6939428 |
K562 cells, H-mLibA CRISPR/Cas9 library, day24, replicate 2 |
| GSM6939429 |
K562 cells, H-mLibB CRISPR/Cas9 library, day0 |
| GSM6939430 |
K562 cells, H-mLibB CRISPR/Cas9 library, day4, replicate 1 |
| GSM6939431 |
K562 cells, H-mLibB CRISPR/Cas9 library, day4, replicate 2 |
| GSM6939432 |
K562 cells, H-mLibB CRISPR/Cas9 library, day10, replicate 1 |
| GSM6939433 |
K562 cells, H-mLibB CRISPR/Cas9 library, day10, replicate 2 |
| GSM6939434 |
K562 cells, H-mLibB CRISPR/Cas9 library, day18 |
| GSM6939435 |
K562 cells, H-mLibB CRISPR/Cas9 library, day24, replicate 1 |
| GSM6939436 |
K562 cells, H-mLibB CRISPR/Cas9 library, day24, replicate 2 |
| GSM6939437 |
Jurkat cells, Brunelllo CRISPR/Cas9 library, day0, replicate 1 |
| GSM6939438 |
Jurkat cells, Brunelllo CRISPR/Cas9 library, day0, replicate 2 |
| GSM6939439 |
Jurkat cells, Brunelllo CRISPR/Cas9 library, day12, replicate 1 |
| GSM6939440 |
Jurkat cells, Brunelllo CRISPR/Cas9 library, day12, replicate 2 |
| GSM6939441 |
Jurkat cells, Brunelllo CRISPR/Cas9 library, day18, replicate 1 |
| GSM6939442 |
Jurkat cells, Brunelllo CRISPR/Cas9 library, day18, replicate 2 |
| GSM6939443 |
Jurkat cells, Brunelllo CRISPR/Cas9 library, day24, replicate 1 |
| GSM6939444 |
Jurkat cells, Brunelllo CRISPR/Cas9 library, day24, replicate 2 |
| GSM6939445 |
Jurkat cells, H-mLibA CRISPR/Cas9 library, day0, replicate 1 |
| GSM6939446 |
Jurkat cells, H-mLibA CRISPR/Cas9 library, day0, replicate 2 |
| GSM6939447 |
Jurkat cells, H-mLibA CRISPR/Cas9 library, day12, replicate 1 |
| GSM6939448 |
Jurkat cells, H-mLibA CRISPR/Cas9 library, day12, replicate 2 |
| GSM6939449 |
Jurkat cells, H-mLibA CRISPR/Cas9 library, day18, replicate 1 |
| GSM6939450 |
Jurkat cells, H-mLibA CRISPR/Cas9 library, day18, replicate 2 |
| GSM6939451 |
Jurkat cells, H-mLibA CRISPR/Cas9 library, day24, replicate 1 |
| GSM6939452 |
Jurkat cells, H-mLibA CRISPR/Cas9 library, day24, replicate 2 |
| GSM6939453 |
Jurkat cells, H-mLibB CRISPR/Cas9 library, day0, replicate 1 |
| GSM6939454 |
Jurkat cells, H-mLibB CRISPR/Cas9 library, day0, replicate 2 |
| GSM6939455 |
Jurkat cells, H-mLibB CRISPR/Cas9 library, day12, replicate 1 |
| GSM6939456 |
Jurkat cells, H-mLibB CRISPR/Cas9 library, day12, replicate 2 |
| GSM6939457 |
Jurkat cells, H-mLibB CRISPR/Cas9 library, day18, replicate 1 |
| GSM6939458 |
Jurkat cells, H-mLibB CRISPR/Cas9 library, day18, replicate 2 |
| GSM6939459 |
Jurkat cells, H-mLibB CRISPR/Cas9 library, day24, replicate 1 |
| GSM6939460 |
Jurkat cells, H-mLibB CRISPR/Cas9 library, day24, replicate 2 |
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| Relations |
| BioProject |
PRJNA924808 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223086_RAW.tar |
28.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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